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This study was conducted to evaluate the inclusion of two levels (2.5 e 5.0%) of dried yeast (Saccharomyces cerevisiae) and its by-products, disrupted yeast cells and yeast cell wall in diets for juveniles of pacu (Piaractus mesopotamicus). Production performance, body and plasmatic composition indexes were evaluated. Seven isoproteic (26% digestible protein) and isoenergetic (3.100 kcal digestible energy) diets were formulated containing increased levels of each ingredient. The diets were supplied for 86 days, "ad libitum". Yeast and by-products increase feed efficiency and protein use, when compared to the control diet. Carcass composition and plasmatic (glucose, cortisol, uric acid, urea and plasmatic protein) levels are not affected by the test... Tipo: Info:eu-repo/semantics/article Palavras-chave: Piaractus mesopotamicus; Pre-biotic; Production performance; Saccharomyces cerevisiae; Yeast by-products. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000300001 Mobilization of endogenous glycogen and trehalose of industrial yeasts BJM Paulillo,Silene Cristina de Lima; Yokoya,Fumio; Basso,Luiz Carlos. The fermentation of yeast reserve carbohydrates, glycogen and trehalose is a procedure to increase protein level of yeast cells and improve ethanol production. This work studied on the degradation kinetics of glycogen and trehalose carried out with two industrial strains of Saccharomyces cerevisiae (PE-2 and SA-1) and the effect of different temperatures (38º, 40º, 42º and 44ºC) on degradation rate. Endogenous fermentation was carried out in a yeast suspension at 20% (w/v) based on wet weight, suspended in fermented media with 3.0 to 4.5 % (v/v) of ethanol. The degradation of the carbohydrate reserves at 40ºC followed first-order kinetics, showing that its rate is mainly dependent on the carbohydrate concentration in the cell. The degradation rate (k)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; Glycogen; Trehalose; Yeast; Fermentation. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300014 Monitoring Saccharomyces cerevisiae populations by mtDNA restriction analysis and other molecular typing methods during spontaneous fermentation for production of the artisanal cachaça BJM Araújo,Roberta A.C.; Gomes,Fátima C.O.; Moreira,Elizabeth S.A.; Cisalpino,Patrícia S.; Rosa,Carlos A.. An ecological study on Saccharomyces cerevisiae populations in spontaneous fermentation has been conducted in three vats of a cachaça distillery in Minas Gerais, Brazil. Ninety-seven yeast isolates were collected at the beginning, the middle and at the end of the production period, and were identified by standard methods. Differentiation between the indigenous S. cerevisiae strains isolated was performed by mitochondrial DNA (mtDNA) restriction analysis, RAPD-PCR, and PCR fingerprint using an intron splice primer. Analysis of the mtDNA restriction profiles revealed 12 different patterns, 11 corresponding to indigenous yeasts (I to XI) and one (XII) to a commercial strain of the bakery yeast. Pattern II (53.6% of the population) and pattern IV strains were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; Cachaça; Fermentation; Molecular diversity. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200006 Nutritive value of diets containing inactive dry yeast for lactating Saanen goats R. Bras. Zootec. Gomes,Ludmila Couto; Alcalde,Claudete Regina; Lima,Larissa Ribas de; Lima,Luciano Soares de; Souza,Rodrigo de; Possamai,Ana Paula Silva. Twenty-four Saanen goats, 15 multiparous and nine primiparous, were distributed in a completely randomised design in a factorial arrangement (3 diets × 2 parities). The treatments were soybean meal (SB), soybean + dry yeast (SBDY), or dry yeast (DY) as a protein source in the diet, and ground corn, mineral supplement, and corn silage (400 g/kg). The study was conducted to evaluate the nutritive value of diets containing inactive dry yeast as a protein source postpartum, postpeak, and during late lactation in dairy goats. To estimate the faecal excretion of indigestible material, neutral detergent fibre was used as an internal marker. The diets did not affect dry matter intake (DMI). However, DMI and nutrient intake were higher for multiparous goats... Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Intake; Parturition order; Saccharomyces cerevisiae; Urea nitrogen. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000100006 Partial purification of glucose-6-phosphate dehydrogenase by aqueous two-phase poly(ethyleneglycol)/phosphate systems BJM Ribeiro,Marcela Zanella; Silva,Daniel Pereira; Vitolo,Michele; Roberto,Inês Conceição; Pessoa-Jr,Adalberto. Glucose-6-phosphate dehydrogenase (G6PDH) is an important enzyme used in biochemical and medical studies and in several analytical methods that have industrial and commercial application. This work evaluated the extraction of G6PDH in aqueous two-phase system (ATPS) of poly(ethyleneglycol) (PEG)/phosphate buffer, using as enzyme source a medium prepared through commercial baker's yeast disruption. Firstly, the effects of PEG molar mass on the enzyme partition and of homogenization and rest on the system equilibrium were investigated. Afterwards, several ATPS were prepared using statistical analysis (2² factorial design). The results, including kinetic and thermodynamic parameters for the G6PDH activity, showed partial purification of this enzyme in ATPS... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aqueous two-phase systems; Glucose-6-phosphate dehydrogenase; Liquid-liquid extraction; Saccharomyces cerevisiae. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100016 Performance of dairy goats fed diets with dry yeast from sugar cane as protein source R. Bras. Zootec. Lima,Luciano Soares de; Alcalde,Claudete Regina; Freitas,Hanna Sakamoto; Molina,Bruna Susan de Labio; Macedo,Francisco de Assis Fonseca de; Horst,José Augusto. The effects of inactive dry yeast (Saccharomyces cerevisiae) from sugar cane were studied in 18 primiparus Saanen dairy goats (51.07±1.43) on dry matter intake and digestibility, milk production and quality. Animals were distributed in a completely randomized design during 90 days (from day 60 of milking). Diets were composed of soybean meal; soybean meal + dry yeast; or dry yeast, as protein sources, and ground corn, mineral supplement and corn silage (40%). Animals fed the dry yeast diet showed lower intake of dry matter (DM), organic matter (OM), crude protein, ether extract and neutral detergent fiber. Diets did not influence milk yield; however the milk production efficiency (kg of milk produced/kg of crude protein ingested) was better in goats fed... Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Goats; Milk quality; Saccharomyces cerevisiae. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000100033 Performance of lactating goats fed diets containing inactive dry yeast R. Bras. Zootec. Gomes,Ludmila Couto; Alcalde,Claudete Regina; Macedo,Francisco de Assis Fonseca de; Santos,Geraldo Tadeu dos; Valloto,Altair Antônio; Lima,Luciano Soares de; Molina,Bruna Susan de Labio. Twenty-four Saanen goats (15 multiparous and 9 primiparous) from 21 days prepartum to 200 days in lactation were used to evaluate milk yield and composition. Animals were randomly distributed in a 3 × 2 factorial arrangement (3 diets × 2 parity orders) and fed a 40:60 forage:concentrate diet composed of soybean meal, soybean meal + dry yeast or dry yeast as protein source, plus ground corn, mineral supplement and corn silage. The protein source did not influence the body weight of pre and postpartum goats. Prepartum, postpartum and postpeak dry matter intake (DMI) were not altered by diets. However, goats fed the diet containing dry yeast as protein source had lower DMI in late lactation. Milk yield and feed efficiency were not affected by diets. Milk... Tipo: Info:eu-repo/semantics/article Palavras-chave: Feed additive; Lactation; Parity; Saccharomyces cerevisiae. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000016 Pharmacology and toxicology of diphenyl diselenide in several biological models BJMBR Rosa,R.M.; Roesler,R.; Braga,A.L.; Saffi,J.; Henriques,J.A.P.. The pharmacology of synthetic organoselenium compounds indicates that they can be used as antioxidants, enzyme inhibitors, neuroprotectors, anti-tumor and anti-infectious agents, and immunomodulators. In this review, we focus on the effects of diphenyl diselenide (DPDS) in various biological model organisms. DPDS possesses antioxidant activity, confirmed in several in vitro and in vivo systems, and thus has a protective effect against hepatic, renal and gastric injuries, in addition to its neuroprotective activity. The activity of the compound on the central nervous system has been studied since DPDS has lipophilic characteristics, increasing adenylyl cyclase activity and inhibiting glutamate and MK-801 binding to rat synaptic membranes. Systemic... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diphenyl diselenide; Organoselenium; Antioxidants; Neuroprotection; Saccharomyces cerevisiae; Mutagenesis. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000001 Photodynamic DNA damage induced by phycocyanin and its repair in Saccharomyces cerevisiae BJMBR Pádula,M.; Boiteux,S.. In the present study, we analyzed DNA damage induced by phycocyanin (PHY) in the presence of visible light (VL) using a set of repair endonucleases purified from Escherichia coli. We demonstrated that the profile of DNA damage induced by PHY is clearly different from that induced by molecules that exert deleterious effects on DNA involving solely singlet oxygen as reactive species. Most of PHY-induced lesions are single strand breaks and, to a lesser extent, base oxidized sites, which are recognized by Nth, Nfo and Fpg enzymes. High pressure liquid chromatography coupled to electrochemical detection revealed that PHY photosensitization did not induce 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) at detectable levels. DNA repair after PHY... Tipo: Info:eu-repo/semantics/article Palavras-chave: Phycocyanin; Saccharomyces cerevisiae; OGG1; Nucleotide excision repair. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900002 Preliminary study of ethanol production from sorghum Thai Agricultural Siwawej, S.; Suraphanpisit, Y.. Palavras-chave: Sorghum; Ethanol; Production; Fermentation; Saccharomyces cerevisiae; Mold; Enzyme activity; ข้าวฟ่าง; เอทานอล; แป้งข้าวฟ่าง; ยีสต์; พันธุ์วาย-90; การผลิต. Ano: 1981 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4081 Probing the SERCA1a sarcoplasmic reticulum Ca2+-ATPase phosphorylation-site mutant D351E with inorganic phosphate BJMBR Carreira,A.C.O.; Bastos,C.M.V.; Verjovski-Almeida,S.. The expression of sarcoplasmic reticulum SERCA1a Ca2+-ATPase wild-type and D351E mutants was optimized in yeast under the control of a galactose promoter. Fully active wild-type enzyme was recovered in yeast microsomal membrane fractions in sufficient amounts to permit a rapid and practical assay of ATP hydrolysis and phosphoenzyme formation from ATP or Pi. Mutant and wild-type Ca2+-ATPase were assayed for phosphorylation by Pi under conditions that are known to facilitate this reaction in the wild-type enzyme, including pH 6.0 or 7.0 at 25ºC in the presence of dimethylsulfoxide. Although glutamyl (E) and aspartyl (D) residue side chains differ by only one methylene group, no phosphoenzyme could be detected in the D351E mutant, even upon the addition of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphorylation site mutant; Catalytic site; Yeast expression system; Saccharomyces cerevisiae; P-type ATPase. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000004 Production and characterization of glucoamylase from fungus Aspergillus awamori expressed in yeast Saccharomyces cerevisiae using different carbon sources BJM Pavezzi,Fabiana Carina; Gomes,Eleni; Silva,Roberto da. Glucoamylase is widely used in the food industry to produce high glucose syrup, and also in fermentation processes for production beer and ethanol. In this work the productivity of the glucoamylase of Aspergillus awamori expressed by the yeast Saccharomyces cerevisiae, produced in submerged fermentation using different starches, was evaluated and characterized physico-chemically. The enzyme presented high specific activity, 13.8 U/mgprotein or 2.9 U/mgbiomass, after 48 h of fermentation using soluble starch as substrate. Glucoamylase presented optimum activity at temperature of 55ºC, and, in the substratum absence, the thermostability was for 1h at 50ºC. The optimum pH of activity was pH 3.5 - 4.0 and the pH stability between 5.0 and 7.0. The half life at... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucoamylase; Aspergillus awamori; Saccharomyces cerevisiae; Corn starch; Potato starch; Cassava starch. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100024 Production of bioethanol using agricultural waste: banana pseudo stem BJM Ingale,Snehal; Joshi,Sanket J.; Gupte,Akshaya. India is amongst the largest banana (Musa acuminata) producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. Present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. Two fungal strains Aspergillus ellipticus and Aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse were used under co-culture fermentation on banana pseudo stem to degrade holocellulose and facilitate maximum release of reducing sugars. The hydrolysate obtained after alkali and microbial treatments was fermented by Saccharomyces cerevisiae NCIM 3570 to produce... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioethanol; Banana pseudostem; Aspergillus ellipticus; Aspergillus fumigatus; Saccharomyces cerevisiae. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300018 Production of ethanol by Saccharomyces cerevisiae and Zymomonas mobilis Coimmobilized: proposal for the use of organic waste Agrociencia Ruiz-Marín,Alejandro; Canedo-López,Yunuén; Narváez-García,Asteria; Robles-Heredia,J. Carlos. Abstract: Fermentation technologies were developed to improve the production of ethanol and an alternative is the immobilization technology, which offers advantages in comparison with free cells culture, such as the possibility of efficiently incorporating symbiotic bacteria in the same matrix. The aim of this study was to evaluate, in equivalent medium, the use of mango (Mangifera indica) waste to produce ethanol. The study compared the production of ethanol by using Zymomonas mobilis and Saccharomyces cerevisiae in free cells culture, immobilized and coimmobilized in calcium alginate in a culture medium equivalent to mango juice. It also included an evaluation of the effect of glucose concentration on productivity in a coimmobilized system. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mangifera indica; Immobilization; Coimmobilization; Ethanol; Zymomonas mobilis; Saccharomyces cerevisiae. Ano: 2016 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952016000500551 Production of ethanol from mesquite (Prosopis juliflora (SW) D.C.) pods mash by Zymomonas mobilis and Saccharomyces cerevisiae Electron. J. Biotechnol. da Silva,Celiane Gomes Maia; Stamford,Tânia Lúcia Montenegro; de Andrade,Samara Alvachian Cardoso; de Souza,Evandro Leite; de Araújo,Janete Magali. This study aimed to assess the use of mesquite pods hydrated mash as biomass for the growth of Saccharomyces cerevisiae UFEPEDA-1012 and Zymomonas mobilis UFEPEDA-205 and for ethanol production using a submerged fermentation. A 2³ factorial design was used to analyze the effects of the type of microorganism, time of fermentation and condition of cultivation on the ethanol production in mesquite pods mash (30 g 100 mL-1). From the obtained results the hydrated mesquite pods mash presented as a good substrate for the growth of S. cerevisiae and Z. mobilis in comparison to the standard media. The effect that most affected the ethanol production was the type of microorganism. The highest ethanol concentration (141.1 gL-1) was found when Z. mobilis was... Tipo: Journal article Palavras-chave: Ethanol; Mesquite; Saccharomyces cerevisiae; Zymomonas mobilis. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500012 Qualidade dos ovos de poedeiras comerciais alimentadas com levedura seca de cana-de-açúcar PAB Maia,George André Rodrigues; Fonseca,José Brandão; Soares,Rita da Trindade Ribeiro Nobre; Silva,Martinho de Almeida e; Souza,Cláudio Luiz Melo de. Este trabalho objetivou avaliar o efeito da adição de levedura seca (Saccharomyces cerevisiae) à dieta de poedeiras na qualidade dos ovos. Foram utilizadas 120 poedeiras comerciais com 33 semanas de idade, distribuídas em um delineamento estatístico de blocos ao acaso, com cinco tratamentos (0, 7, 14, 21 e 28% de levedura), quatro repetições e seis aves por unidade experimental. Rações isoprotéicas (18% de proteína bruta), isoenergéticas (2.800 kcal de energia metabolizável/kg), isocálcicas (3,8% Ca) e isofosfóricas (0,38% P disponível) foram formuladas à base de milho e farelo de soja. Os níveis de levedura seca não alteraram o peso do ovo (64,35± 0,85 g), a altura do albúmen (7,95±0,22 mm), a unidade Haugh (87,42±1,31), os sólidos totais da gema... Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; Alimento; Nutrição animal; Ave. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002000900013 Quantitative characteristics of carcass and meat quality of ¾ Boer + ¼ Saanen and Saanen goat kids fed diets with dry yeast R. Bras. Zootec. Freitas,Hanna Sakamoto; Alcalde,Claudete Regina; Lima,Luciano Soares de; Macedo,Francisco de Assis Fonseca de; Macedo,Vicente de Paulo; Molina,Bruna Susan de Labio. Twenty-seven carcasses of non-castrated confined goat kids, (18 ¾ Boer + ¼ Saanen and nine Saanen) with slaughtered weight of 30.1 ± 0.27 kg were used to evaluate quantitative characteristics of the carcass and qualitative characteristics of the loin and shoulder. The animals were distributed in a completely randomized design in a 2 × 3 factorial arrangement (breed group × diet). The diets consisted of inclusion of dry yeast, as follows: soybean meal, soybean meal + dried yeast or dried yeast. The other ingredients of the diets were oat hay (30% DM), ground corn and mineral mixture. Hot carcass weight, cold carcass weight, weight loss by cooling, true carcass yield and carcass compactness index were not influenced by the inclusion of dry yeast nor by... Tipo: Info:eu-repo/semantics/article Palavras-chave: Byproduct of sugar cane; Genetic group; Goat; Meat quality; Saccharomyces cerevisiae. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000300023 Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification BABT Silva,Gildo Almeida da; Bernardi,Taís Letícia; Schaker,Patrícia Dayane Carvalho; Menegotto,Morgana; Valente,Patricia. The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive. Tipo: Info:eu-repo/semantics/article Palavras-chave: Boiling; Freeze-thawing; DNA extraction; Dekkera bruxellensis; Saccharomyces cerevisiae. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200020 Residual polysaccharides from fungi reduce the bacterial spot in tomato plants Bragantia Aguiar,Tarsis; Luiz,Caroline; Rocha Neto,Argus Cezar; Di Piero,Robson Marcelo. Abstract Polysaccharides from fungal wastes were partially characterized and evaluated for their protective effects against bacterial spot caused by Xanthomonas gardneri on four tomato cultivars: Santa Cruz Kada, Natália, BRS Sena and Forty. The polysaccharides were extracted from spent mushroom substrate of Pleurotus ostreatus, residual brewery yeast (Saccharomyces cerevisiae), and basidiocarps discarded from Lentinula edodes production. These polysaccharides were characterized for total carbohydrates, phenolics and proteins content, pH, scatter intensity, conductivity, Zeta potential, DPPH scavenging assay and infrared spectroscopy. The effects of time interval between treatment and inoculation (4 or 7 days) and polysaccharide concentrations (0.5 or 1.5... Tipo: Info:eu-repo/semantics/article Palavras-chave: Induced resistance; Lentinula edodes; Pleurotus ostreatus; Saccharomyces cerevisiae; Solanum lycopersicon; Xanthomonas gardneri. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000200299 RNA-binding proteins and their role in the regulation of gene expression in Trypanosoma cruzi and Saccharomyces cerevisiae Genet. Mol. Biol. Oliveira,Camila; Faoro,Helisson; Alves,Lysangela Ronalte; Goldenberg,Samuel. Abstract RNA-binding proteins (RBPs) have important functions in the regulation of gene expression. RBPs play key roles in post-transcriptional processes in all eukaryotes, such as splicing regulation, mRNA transport and modulation of mRNA translation and decay. RBPs assemble into different mRNA-protein complexes, which form messenger ribonucleoprotein complexes (mRNPs). Gene expression regulation in trypanosomatids occurs mainly at the post-transcriptional level and RBPs play a key role in all processes. However, the functional characterization of RBPs in Trypanosoma cruzi has been impaired due to the lack of reliable reverse genetic manipulation tools. The comparison of RBPs from Saccharomyces cerevisiae and T. cruzi might allow inferring on the function... Tipo: Info:eu-repo/semantics/article Palavras-chave: RNA-binding proteins; Trypanosoma cruzi; Saccharomyces cerevisiae; Gene expression regulation. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100022 Registros recuperados: 128 Primeira ... 1 2 3 4 5 6 7 ... Última

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